Figure 2
From: Transformation of Epichloë typhina by electroporation of conidia
Sensitivity of Epichloë typhina conidia to hygromycin B. Selection of untransformed conidia (5000 conidia/plate) on increasing concentrations of hygromycin B on Corn Meal Malt Agar (CMMA). As shown above, E. typhina conidia demonstrated resistance to hygromycim B. Based on this analysis we decided to use 200 μg/mL and 300 μg/mL of hygromycin B for selection of transformants.