Cyclic stretch increases splicing noise rate in cultured human fibroblasts

BMC Research Notes

Table 3 Relative occurrence of the exon skip transcripts (ΔCT) in cultured fibroblasts.

Gene/exon	CT (WT)	CT (skip)	ΔCT	(%)
AATF-Δ3	23.022 ± 0.130	39.630 ± 1.041	16.607 ± 1.049	(0.001%)
MAP3K11-Δ9	24.228 ± 0.095	37.384 ± 0.041	13.156 ± 0.103	(0.010%)
NF1-Δ38	23.221 ± 0.083	27.377 ± 0.096	4.156 ± 0.127	(5.609%)
NF1-Δ39	22.615 ± 0.045	34.883 ± 0.220	12.267 ± 0.225	(0.020%)
PCGF2-Δ10	23.214 ± 0.201	38.793 ± 1.311	15.579 ± 1.326	(0.002%)
POLR2A-Δ23	24.408 ± 0.205	39.826 ± 0.350	15.419 ± 0.405	(0.002%)
RABAC1-Δ4	22.304 ± 0.131	29.750 ± 0.123	7.446 ± 0.180	(0.573%)

CT of the skip trancripts of AATF-Δ3, MAP3K11-Δ9, NF1-Δ38, NF1-Δ39, PCGF2-Δ10, POLR2A-Δ23 and RABAC1-Δ4 in relation to CT of the wildtype transcripts (number of measurements n = 3, for exon skip n = 4) in cultured fibroblasts (K14). CT values for wildtype (WT), the exon skip transcripts (skip) and the difference (ΔCT) (SD) and the percentage (%) of the amount of the exon skip product relative to the wildtype product.

ISSN: 1756-0500