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Figure 1 | BMC Research Notes

Figure 1

From: Variability of protein level and phosphorylation status caused by biopsy protocol design in human skeletal muscle analyses

Figure 1

Western blot analysis strategy exemplified for phospho-Akt. A) To control for the non-linearity between Western blot signal and the amount of protein loaded, a dilution series of protein extracts obtained from human primary myoblasts was loaded on every gel in order to build a calibration curve. B) Each volume of myoblasts protein extract was plotted against their optical density. Optical densities of subject's muscle extracts were then reported on the calibration curve and the corresponding volume of protein extract was found. C) These data were considered the standardized values and were used for the final analysis. p-Akt = phospho-Akt

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