Figure 3

Akt, GSK-3β, 4E-BP1 and p70 S6K phosphorylation levels are influenced by muscle biopsy conditions. All biopsies of each subject were blotted against phospho-Akt, phospho-GSK-3β, phospho-4E-BP1, phospho-p70 S6K. Standardized value for every band optical density was obtained and protein expression level between biopsy conditions were compared as shown in bar graphs. All results were normalized with tubulin signal to ensure equal loading. Data are expressed in percentage of variation between two biopsy situations. On the left side, data are expressed as real values where positive and negative variation levels are depicted. The vertical line represents the mean variation level. On the right side, every difference between compared biopsies was transformed in absolute values, so that no distinction was made between an increase and a decrease in protein expression between two conditions. Results are expressed as mean ± SEM of 6 intra-subject variations. p- = phosphorylated