Table 1 Western blot technique-induced variability of the duplicates per protein.
Protein tested | Technique-induced variability |
|---|---|
Akt | 27% |
phospho-Akt | 25% |
GSK-3β | 26% |
phospho-GSK-3β | 25% |
p70 S6K | 20% |
phospho-p70 S6K | 50% |
MuRF1 | 37% |
phospho-4E-BP1 | 48% |
Total proteins | 28% |
Phospho-proteins | 37% |
Overall | 32% |