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Table 1 Western blot technique-induced variability of the duplicates per protein.

From: Variability of protein level and phosphorylation status caused by biopsy protocol design in human skeletal muscle analyses

Protein tested Technique-induced variability
Akt 27%
phospho-Akt 25%
GSK-3β 26%
phospho-GSK-3β 25%
p70 S6K 20%
phospho-p70 S6K 50%
MuRF1 37%
phospho-4E-BP1 48%
Total proteins 28%
Phospho-proteins 37%
Overall 32%
  1. 4E-BP1, eukaryotic translation initiation factor 4E binding protein 1; GSK-3β, Glycogen synthase kinase-3 beta; p70 S6K, p70 S6 kinase; MuRF1, Muscle RING finger 1.