Immunohistochemistry tissue microarray (TMA) of specimens of human larynx. Immunostaining of the S100A7 protein using the anti-recombinant S100A7 (His-tag) rabbit serum at 1:12,000 dilution. a and b show the negative control (absence of primary antibody) for IHC. c and d, low staining (cytoplasm), spot number (417595 T2) containing squamous cell carcinoma with a 1+ staining score (same spot number). e and f, moderate staining (cytoplasm), spot number (426088 G2) containing squamous cell carcinoma. g and h, strong staining (cytoplasmic and nuclear localization); spot number (426088 T2) containing squamous cell. i and j, strong staining (cytoplasmic and nuclear localization); spot number (417739 T2) containing squamous cell carcinoma. The panels with the letters A, C, E, G and were magnified ×100, and the panels with the letters B, D, F, H and J were magnified ×400. The brownish red region indicates the immunoreactivity of the rabbit polyclonal antibody with the human S100A7 protein expressed in the tissue of the larynx with neoplasia, after being labeled with a chromogenic substrate (DAB) using the NovoLink Polymer Detection System kit (Novocastra, Newcastle Upon Tyne, UK).