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Table 1 Classification of wild boar sera through five freeze-thaw cycles.

From: Effect of haemolysis and repeated freeze-thawing cycles on wild boar serum antibody testing by ELISA

  Clean Haemolysed
Freeze/thaw cycle 1 2 3 4 5 1 2 3 4 5
Sample number           
1 1 1 1 1 1 1 1 1 1 1
2 1 1 1 1 1 1 1 1 1 1
3 0 0 0 0 0 0 0 0 0 2
4 0 0 0 0 2 0 0 0 0 2
5 0 0 0 0 0 0 0 0 0 0
6 1 1 1 1 1 1 1 1 1 1
7 1 1 1 1 1 1 1 1 1 1
8 0 0 0 0 0 0 0 2 0 2
9 1 1 1 1 1 0 0 0 2 1
10 1 1 1 1 1 1 1 1 1 1
11 0 0 0 0 0 1 1 1 1 1
12 1 1 1 1 1 1 1 1 1 1
13 0 2 0 0 0 2 2 0 0 0
14 1 1 1 1 1 1 1 1 1 1
15 0 0 0 0 0 0 0 0 0 2
16 1 1 1 1 1 1 1 1 1 1
17 0 0 0 0 0 0 0 0 2 2
18 0 0 0 0 2      
19 0 0 0 0 0      
20 1 1 1 1 1      
N° positive samples 10 10 10 10 10 9 9 9 9 10
N° negative samples 10 9 10 10 8 7 7 7 6 2
N° doubtful samples 0 1 0 0 2 1 1 1 2 5
N° changing state   1 0 0 2   0 2 3 7
% changing state   5% 0% 0% 10%   0% 12% 18% 41%
  1. Classification of the 20 wild boar sera samples (20 clean; 17 haemolysed) after 1 to 5 freeze-thaw cycles into positive (1), negative (0) or doubtful (2) according to a commercial ELISA for the detection of serum immunoglobulin G antibodies against Aujeszky's disease virus.