Table 1 Classification of wild boar sera through five freeze-thaw cycles.
From: Effect of haemolysis and repeated freeze-thawing cycles on wild boar serum antibody testing by ELISA
Clean | Haemolysed | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
Freeze/thaw cycle | 1 | 2 | 3 | 4 | 5 | 1 | 2 | 3 | 4 | 5 |
Sample number | ||||||||||
1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
4 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 2 |
5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
6 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
7 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 |
9 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 2 | 1 |
10 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
11 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 |
12 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
13 | 0 | 2 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 0 |
14 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
15 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
16 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
17 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 |
18 | 0 | 0 | 0 | 0 | 2 | |||||
19 | 0 | 0 | 0 | 0 | 0 | |||||
20 | 1 | 1 | 1 | 1 | 1 | |||||
N° positive samples | 10 | 10 | 10 | 10 | 10 | 9 | 9 | 9 | 9 | 10 |
N° negative samples | 10 | 9 | 10 | 10 | 8 | 7 | 7 | 7 | 6 | 2 |
N° doubtful samples | 0 | 1 | 0 | 0 | 2 | 1 | 1 | 1 | 2 | 5 |
N° changing state | 1 | 0 | 0 | 2 | 0 | 2 | 3 | 7 | ||
% changing state | 5% | 0% | 0% | 10% | 0% | 12% | 18% | 41% | ||