Figure 2

AITC induces expression of HSP70 in C. elegans when treated with 0-10 μM AITC for 2 h at room temperature. (a) Human HSP70 (Accession No. NM_005346), residues 429-480, show 98% homology with C. elegans HSP70A (Accession No. M18540). A monoclonal antibody directed against a synthetic peptide for this region of the human protein was obtained to detect the C. elegans homologue (b) Increasing concentrations of AITC induce greater expression of HSP70, indicated with arrowhead. Proteins in cell lysates from AITC treated C. elegans cultures were separated by SDS-PAGE and HSP70 protein detected using the anti-HSP70 monoclonal antibody. (c) Increasing concentrations of AITC induce HSP70A mRNA expression in C. elegans, as determined by qRT-PCR. Bars on the columns represent standard deviations of three values. Data was analyzed by one-way ANOVA. Values marked with different letters were significantly (p < 0.01).