Differential production of proteins in M. haemolytica ΔNarP7 in response to NaNO
supplementation in the media, shown by ISO-DALT 2D electrophoresis. A 900 μg of total protein preparations from SH1217 grown with or without NaNO3 supplementation were separated by isoelectric focusing on a 17 cm IEF strip. The proteins were separated in the second dimension by SDS-PAGE and visualized by Colloidal Coomassie blue staining. None of these spots were shown to be differentially produced in ΔNarP7 under the two NaNO3 conditions. The spot used for normalization in statistical analysis is labeled 'N'.