Table 1 Proteins differentially produced in response to NaNO3 supplementation determined by the large-format ISO-DALT 2D electrophoresis
Spot # | Differential production in SH1217a | Differential production in ΔNarP7 | Identification (accession number) | MS Coverage (%) |
|---|---|---|---|---|
2 | 2.17 | no response | 50S ribosomal protein RplI (197748946) | 52 |
3 | 1.82 | no response | Ribosomal protein S6 (53729064) | 53 |
4/5 | 1.88/1.69 | no response | Peptide chain release factor RF4/ribosome recycling factor (RRF) (197750015) | 51/26 |
8 | 2.47 | no response | Manganese dependent superoxide dismutase (51292145) | 25 |
9 | 1.72 | no response | Xanthine-guanine phosphorybosyltransferase (113460152) | 15 |
11 | 1.64 | no response | 3-oxoacyl-[ACP]-reductase (197747648) | 38 |
13 | 2.19 | no response | Hypothetical protein (OmpH?) (197749776) | 8 |
14/18/26/27 | 4.09/3.7/only in SH+/only in SH+ | no response | Iron (Fe3+) ABC Transporter, FbpA (197750010) | 37/31/29/30 |
16/19 | 2.66/3.13 | no response | Iron Transporter, YfeA (110735183) | 38/29 |
22/23 | 2.75/2.93 | no response | Glyceraldehyde-3-phosphate dehydrogenase (197749950) | 29/29 |
24 | 3.29 | no response | Aspartate transaminase (197749486) | 24 |
28/29 | 0.27/0.34 | no response | Phosphophenolpyruvate carboxykinase (197749783) | 30/21 |
30 | 0.34 | no response | 2',3'-cyclic-nucleotide 2'-phosphodiesterase (197747925) | 13 |
31 | 0.32 | no response | Phosphoglycerate kinase (197750198) | 55 |
32 | Only in SH - | no response | Elongation factor EF1B (197750012) | 36 |
33 | Only in SH - | no response | PTS family mannose porter component IIAB (197748528) | 9 |
34 | Only in SH - | no response | L-asparaginase II (197747589) | 19 |