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Table 1 Proteins differentially produced in response to NaNO3 supplementation determined by the large-format ISO-DALT 2D electrophoresis

From: A proteomic analysis of the regulon of the NarP two-component regulatory system response regulator in the bovine pathogen Mannheimia haemolytica A1

Spot # Differential production in SH1217a Differential production in ΔNarP7 Identification (accession number) MS Coverage (%)
2 2.17 no response 50S ribosomal protein RplI (197748946) 52
3 1.82 no response Ribosomal protein S6 (53729064) 53
4/5 1.88/1.69 no response Peptide chain release factor RF4/ribosome recycling factor (RRF) (197750015) 51/26
8 2.47 no response Manganese dependent superoxide dismutase (51292145) 25
9 1.72 no response Xanthine-guanine phosphorybosyltransferase (113460152) 15
11 1.64 no response 3-oxoacyl-[ACP]-reductase (197747648) 38
13 2.19 no response Hypothetical protein (OmpH?) (197749776) 8
14/18/26/27 4.09/3.7/only in SH+/only in SH+ no response Iron (Fe3+) ABC Transporter, FbpA (197750010) 37/31/29/30
16/19 2.66/3.13 no response Iron Transporter, YfeA (110735183) 38/29
22/23 2.75/2.93 no response Glyceraldehyde-3-phosphate dehydrogenase (197749950) 29/29
24 3.29 no response Aspartate transaminase (197749486) 24
28/29 0.27/0.34 no response Phosphophenolpyruvate carboxykinase (197749783) 30/21
30 0.34 no response 2',3'-cyclic-nucleotide 2'-phosphodiesterase (197747925) 13
31 0.32 no response Phosphoglycerate kinase (197750198) 55
32 Only in SH - no response Elongation factor EF1B (197750012) 36
33 Only in SH - no response PTS family mannose porter component IIAB (197748528) 9
34 Only in SH - no response L-asparaginase II (197747589) 19
  1. afold-change
  2. The spots were identified by liquid chromatography-mass spectrometry. The spot number corresponds to Figures 2, 3, 4 and 5. Some spots represented the same protein. Differential production of the spots is shown by the following ratio: [volume of the spot when grown with additional NaNO3/volume of the spot when grown without additional NaNO3]. Some spots were produced only when SH1217 was grown with NaNO3 supplementation (Only in SH+) or without NaNO3 supplementation (Only in SH-). The change in the volume of these spots in response to NaNO3 supplementation was not observed in ΔNarP7 (no response)
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