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Figure 1 | BMC Research Notes

Figure 1

From: Visualization and quantitation of the expression of microRNAs and their target genes in neuroblastoma single cells using imaging cytometry

Figure 1

Analysis of the expression of miR-124 and CDK6 in neuroblastoma cells transfected with control miRNA (a) and miR-124 (b). The cells were transfected and stained for miR-124 and CDK6 as described in Materials and Methods. The relative fluorescence intensity level for miR-124-FITC (x-axes) vs. CDK6-AF660 (y-axes) is shown in (a) and (b). Representative images of cells from "control" cell subpopulations-miR-124low/intCDK6hi (gate R1); miR-124lowCDK6low(gate R2); miR-124intCDK6low (gate R3); and miR-124hiCDK6low (gate R4)-are shown in (c). The data is representative of four separate experiments. The average percentages for subsets from gates R1-R4 is shown in (d). In (c) the expression of miR-124 is shown in green and the expression of CDK6 is shown in red. Merged images show the expression of both CDK6 and miR-124. Three representative images are shown for each subset

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