Figure 2

Comparison of the level of proliferation of neuroblastoma cells transfected with control miRNA (a) and miR-124 (b). The cells were transfected and proliferation was assessed by the BrdU incorporation assay as described in Materials and Methods. The cell size (area of the cell) and the BrdU-FITC fluorescence intensity are shown along the x-axis and y-axis, respectively, in (a) and (b). There representative experiment is shown in (a and b) and mean ± S.E. of three separate experiments is shown in (c). Representative images of BrdU^- (negative) and BrdU⁺ (positive) cells of small size (gate R1) and large size (gate R2) are shown in (c). In (c) the expression of BrdU is shown in green and the bright field image of the cells is shown in grey. Three representative images are shown for each subset. In (d), the bright field images of the cells are shown in grey (left images), the expression of BrdU is shown in green (middle images) and merged images are shown on the right. Three representative images are shown for each subset