Identification of superior reference genes for data normalisation of expression studies via quantitative PCR in hybrid roses (Rosa hybrida)

Table 1 The description of the rose candidate reference genes.

Gene	GenBank Accession		Primer sequence (5'-3')	Position	Amplicon	Efficiency
EF1-α (elongation factor 1α)	JN399225	F R	ACACCTCCCACATTGCTGTTA CTTCAAGAACTTGGGCTCCTT	1055-1075 1129-1149	95 nt	0.98
GAPDH (glyceraldehyde-3-phosphate dehydrogenase)	JN399220	F R	TATGACCAGATCAAGGCTGCT ACCAATGAAGTCGGTTGACAC	769-789 850-870	102 nt	0.97
PP2A (protein phosphatase 2A)	JN399224	F R	TGTCACTGCATCAAAGGACAG GACGAATTGTCTTCTCCACCA	1557-1577 1646-1666	110 nt	0.98
SAND (SAND-family protein)	JN399228	F R	GTGTTGAGGAGTTGCCTCTTG AACCTGTCGGGAGAATCTGTT	830-850 906-926	97 nt	0.98
TIP (TIP41-like protein)	JN399221	F R	GAATCCACGGCTGGGAAA CAGTTCGTGGGTGGAGGAGTT	77-94 121-141	65 nt	0.93
Tubulin	JN399223	F R	GTACATGGCCTGCTGTTTGAT ATGGTACGCTTGGTCTTGATG	900-920 969-989	90 nt	0.99
UBC (ubiquitin conjugating protein)	JN399227	F R	GCCAGAGATTGCCCATATGTA TCACAGAGTCCTAGCAGCACA	360-380 448-468	109 nt	0.97

The sequences were derived from a collection of 44343 ESTs generated by 454 sequencing of cDNAs from healthy and stressed rose leaves available in the lab. The primer sequences, their positions, the length of their product and their reaction efficiency in quantitative PCR (qPCR) as estimated by the LRE analyser are given.

ISSN: 1756-0500