Identification of superior reference genes for data normalisation of expression studies via quantitative PCR in hybrid roses (Rosa hybrida)

Table 3 Ranking of the reference gene candidates by BestKeeper, geNorm, NormFinder and RankAggreg.

Rank position	Whole dataset				Different tissues				Stressed leaves
	Best-Keeper	geNorm (M)	NormFinder (Stab. val.)	Rank-Aggreg	Best-Keeper	geNorm (M)	NormFinder (Stab. val.)	Rank-Aggreg	Best-Keeper	geNorm (M)	NormFinder (Stab. val.)	Rank-Aggreg
1	UBC	UBC 0.54	PP2A 0.19	UBC	UBC	PP2A 0.48	PP2A 0.14	PP2A	GAPDH	SAND 0.44	PP2A 0.11	SAND
2	GAPDH	PP2A 0.55	UBC 0.21	PP2A	PP2A	UBC 0.51	UBC 0.18	UBC	UBC	TIP 0.45	UBC 0.12	UBC
3	PP2A	SAND 0.6	SAND 0.27	SAND	SAND	GAPDH 0.56	GAPDH 0.24	GAPDH	TIP	UBC 0.47	SAND 0.12	TIP
4	TIP	TIP 0.62	EF1-α 0.31	TIP	Tubulin	Tubulin 0.59	TIP 0.27	Tubulin	PP2A	EF1-α 0.49	TIP 0.19	PP2A
5	SAND	EF1-α 0.66	GAPDH 0.33	GAPDH	GAPDH	TIP 0.59	Tubulin 0.28	TIP	SAND	PP2A 0.51	EF1-α 0.24	EF1-α
6	EF1-α	GAPDH 0.68	TIP 0.33	EF1- α	EF1-α	SAND 0.61	SAND 0.3	SAND	EF1-α	GAPDH 0.66	Tubulin 0.33	GAPDH
7	Tubulin	Tubulin 0.81	Tubulin 0.4	Tubulin	TIP	EF1-α 0.66	EF1-α 0.33	EF1- α	Tubulin	Tubulin 0.67	GAPDH 0.37	Tubulin
Best comb.		SAND/UBC 0.39	EF1-α/UBC 0.15			SAND/TIP 0.37	TIP/Tubulin 0.08			SAND/TIP 0.27	SAND/UBC 0.07

The analyses were conducted for either the whole dataset, different rose tissues (leaves, flower buds and roots) or for stressed leaves. The best comb. is the proposed best combination of two genes given by the respective algorithm.

ISSN: 1756-0500