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Table 3 Ranking of the reference gene candidates by BestKeeper, geNorm, NormFinder and RankAggreg.

From: Identification of superior reference genes for data normalisation of expression studies via quantitative PCR in hybrid roses (Rosa hybrida)

Rank
position
Whole dataset Different tissues Stressed leaves
  Best-Keeper geNorm (M) NormFinder (Stab. val.) Rank-Aggreg Best-Keeper geNorm (M) NormFinder (Stab. val.) Rank-Aggreg Best-Keeper geNorm (M) NormFinder (Stab. val.) Rank-Aggreg
1 UBC UBC
0.54
PP2A
0.19
UBC UBC PP2A
0.48
PP2A
0.14
PP2A GAPDH SAND
0.44
PP2A
0.11
SAND
2 GAPDH PP2A
0.55
UBC
0.21
PP2A PP2A UBC
0.51
UBC
0.18
UBC UBC TIP
0.45
UBC
0.12
UBC
3 PP2A SAND
0.6
SAND
0.27
SAND SAND GAPDH
0.56
GAPDH
0.24
GAPDH TIP UBC
0.47
SAND
0.12
TIP
4 TIP TIP
0.62
EF1-α
0.31
TIP Tubulin Tubulin
0.59
TIP
0.27
Tubulin PP2A EF1-α
0.49
TIP
0.19
PP2A
5 SAND EF1-α
0.66
GAPDH
0.33
GAPDH GAPDH TIP
0.59
Tubulin
0.28
TIP SAND PP2A
0.51
EF1-α
0.24
EF1-α
6 EF1-α GAPDH
0.68
TIP
0.33
EF1- α EF1-α SAND
0.61
SAND
0.3
SAND EF1-α GAPDH
0.66
Tubulin
0.33
GAPDH
7 Tubulin Tubulin
0.81
Tubulin
0.4
Tubulin TIP EF1-α
0.66
EF1-α
0.33
EF1- α Tubulin Tubulin
0.67
GAPDH
0.37
Tubulin
Best
comb.
  SAND/UBC
0.39
EF1-α/UBC
0.15
   SAND/TIP
0.37
TIP/Tubulin
0.08
   SAND/TIP
0.27
SAND/UBC
0.07
 
  1. The analyses were conducted for either the whole dataset, different rose tissues (leaves, flower buds and roots) or for stressed leaves. The best comb. is the proposed best combination of two genes given by the respective algorithm.