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Table 1 Description of SSR markers developed for Urochloa humidicola (Rendle) Morrone & Zuloaga

From: New microsatellite markers developed from Urochloa humidicola (Poaceae) and cross amplification in different Urochloa species

      

Characteristics in five species*

Characteristics in U. humidicola

SSR Locus

Genbank accession number

Repeat Motif

Ta (°C)

Predicted product size (pb)

Primer Sequences (5'- 3')

N° of bands

Size range (pb)

PIC

N° of bands

PIC

DP

BhUNICAMP028

JF812604

(GT)4GG(GT)3

57

228

TCTTTTGGCTCTGAATGTGCT

TTGATGCCGAATGGAACC

6

195-212

0.7364

5

0.6845

0.9324

BhUNICAMP029

JF812605

(TC)3(TG)8

55

176

AAGGGATATTTGTGTTTCT

TTTTTCAGGATTGCTAAG

7

141-180

0.7363

4

0.6492

0.8708

BhUNICAMP030

JF812606

(AT)3TGC(AT)4

60

133

GGAATATTGTTGCTGAGAGTGG

GCGACGACAGAATAAAAATGAT

3

135-181

0.2211

2

0.1638

0.2123

BhUNICAMP031

JF812607

(GT)7

60

126

AGGATTTAAAGGACCCACCAT

TCCGCTCGGACTGTGATT

3

126-131

0.3749

3

0.3749

0.6554

BhUNICAMP032

JF812608

(GT)7

60

212

GCATATGCAGAGTTTTTGTT

TGACCATTTTTCTTATCTTTCT

10

196-222

0.8305

10

0.8305

0.9292

BhUNICAMP033

JF812609

(TG)5GG(TG)3

60

255

TGAGGTCTTCCGTTCTTGTAGGT

ACGAGGCTGCCCGAGTAATC

5

212-285

0.6965

4

0.6356

0.8246

BhUNICAMP034

JF812610

(GA)20

51

204

TGTAGTGTTGCTGTAGAGTTA

CATTGTTTTGAAGATTTG

3

176-182

0.5894

3

0.5916

0.2800

BhUNICAMP035

JF812611

(AC)4(CA)5CG(CA)3...(CA)5

51

248

GATGCATCTCCCTCCCTTACTC

AGACATTCATCCCGTTTCACAA

15

160-263

0.8767

13

0.8666

0.9784

BhUNICAMP036

JF812612

(TTG)9(TG)6TT(TG)5CG(TG)5

60

265

CGATAGTTAGGCGAGAGTTTTG

TATTGTCGTATGGCAGAGTTCA

9

204-282

0.8127

8

0.8052

0.8800

BhUNICAMP037

JF812613

(TG)8

55

277

CCGTGGAATCCGACAGGTAG

CCGGGAGGAGAGTTAGAAGATG

21

118-302

0.9270

19

0.9238

0.9846

BhUNICAMP038

JF812614

(AG)13

60

294

TCTCTTAAGCGACCAGTA

CAGGAGATAAGTAAAATGAAT

14

286-321

0.8955

14

0.8975

0.9785

BhUNICAMP039

JF812615

(TC)9...(TC)10

55

231

CATACTTGCATTCTTTTGAT

TGTATGAATTTATTGTTTGACT

22

183-263

0.9252

21

0.9254

0.9938

BhUNICAMP040

JF812616

(CTTG)3

60

257

TGTAAGCATATCATTTCGTCTA

ACTGCCCTGTTTTCCTG

2

258-261

0.2772

2

0.2784

0.4092

BhUNICAMP041

JF812617

(TC)3(AAAAT)3

62

178

GCTAGGATGGTGGGCTGTGCT

CGACGTTTCCGGAATGATGTTT

6

173-270

0.4797

3

0.3966

0.5662

BhUNICAMP042

JF812618

(TG)7

60

223

CCGCTGAGCTGTATAGGAAGTT

AAGAGGCGGGACATTTAGGA

6

210-226

0.6465

6

0.6465

0.8400

BhUNICAMP043

JF812619

(GT)3(TG)5GG(TG)4

60

216

TGTGTTTGTGTTCTCTATGTGT

TATGTGATCCAAAAGTGAAGTG

5

212-224

0.697

5

0.7367

0.9046

BhUNICAMP044

JF812620

(CA)11

60

132

TAACACAAGTGCAGGGCGTAAT

TGAACACAGCGACACAAGACAC

17

96-130

0.8913

17

0.8918

0.9815

BhUNICAMP045

JF812621

(AC)11

60

245

ACACCACACCAAATTCTTACCC

TCTCGTTTCATGGCACTGTCTA

14

225-300

0.8956

14

0.8994

0.9600

BhUNICAMP046

JF812622

(TG)10

60

262

ACGCCGCAGCAGTTGGTG

TCAGGACGTGCCGATGGTAAT

22

230-284

0.9268

20

0.9192

0.9877

BhUNICAMP047

JF812623

(TC)20

57

284

TACATGCAGCAACTAAGATA

GCACTAACAAGAAAAGATT

26

211-330

0.9231

23

0.9307

0.9969

BhUNICAMP048

JF812624

(AG)20

57

286

GCACTAAACAAGAAAAGATT

TACATGCAGCAACTAAGATA

29

212-350

0.8864

23

0.9200

0.9754

BhUNICAMP049

JF812625

(AG)3A(AG)4

60

285

GGGCCCGGCACAACAGTAG

AGGCCCACACGCAGAGAACA

8

184-287

0.5597

2

0.3698

0.4277

BhUNICAMP050

JF812626

(TGCG)3

60

236

GTGTGGTTGCAGGACGGATTG

TGAGTGCATGACAGGTGACGAA

7

225-263

0.7144

6

0.7037

0.9692

BhUNICAMP051

JF812627

(AC)17GC(AC)6AT(AG)7

60

294

TAGCAATGCATGGATAAGACT

TGGAGCTCACCCTAAGAAG

1

297

0

1

0

0

BhUNICAMP052

JF812628

(TG)5

60

268

ATAACACGGCCAGAACTA

ATGAACAATCGGGGTAT

12

230-280

0.8663

12

0.8622

0.9446

BhUNICAMP053

JF812629

(CA)12CG(CA)9

60

291

GAGTAAGCTTCTAGGACAGGAT

GCTCAAACAACTCGATAATAAC

18

224-320

0.8887

10

0.8603

0.7692

BhUNICAMP054

JF812630

(TG)9

60

230

CCATATGTGAAGGCTGCGTGAA

GTGGCGGGCTAGTGGCTTATC

15

190-290

0.8262

14

0.8281

0.9385

BhUNICAMP055

JF812631

(TC)7

60

261

GGAAAAAGAAAAGCGGACTGAA

ACGCAAAATAAATGGCAATGGA

12

240-310

0.8829

10

0.8578

0.9538

BhUNICAMP056

JF812632

(TGTT)4

60

239

GCCACAACACGCAAATC

ATGTATGAGGACCCAAGTTATG

4

234-249

0.269

4

0.2841

0.2861

BhUNICAMP057

JF812633

(AG)22

60

219

AGCGACCTCCAGCAACCTT

TTTCCCACTCTTTCCCTCTCAC

21

169-239

0.9091

21

0.9091

0.9846

BhUNICAMP058

JF812634

(TC)18

55

279

CTAAACAGGTAAACAGACAAT

CAAAACGTGAATACATAACA

9

250-340

0.7913

9

0.7913

0.9015

BhUNICAMP059

JF812635

(ATGT)3

55

290

CAATCCATTTTAACAAGAAGTC

GCAACAGTCCGTAGTAAGTATC

5

288-305

0.5747

5

0.5783

0.5538

BhUNICAMP060

JF812636

(TTTTG)3

55

279

AATCCAAAGTCATCCCCACAAT

TTTTTCGGCAACAGACAGGTAA

8

270-290

0.7747

7

0.7721

0.9415

BhUNICAMP061

JF812637

(GT)14

60

165

TGATTCAAAACGCCACGATAGG

GGACCGGAACACTGCTTACGA

22

147-192

0.9139

19

0.9067

0.9846

BhUNICAMP062

JF812638

(CA)8

60

155

CAAACCTCGTGCTCGTG

AGATGGGTTCGGCTGTC

18

139-193

0.8919

15

0.8779

0.9631

BhUNICAMP063

JF812639

(GA)8G(GA)14

60

199

CAAGAAAGCGCGATGAAAAA

GAACACAATGGAGAAGCAG GTC

14

173-230

0.8967

14

0.8967

0.9508

BhUNICAMP064

JF812640

(TC)19

60

175

CCCCTACTTTTATACGACACAT

GAACGAGAGTAGTAGCATTGGT

13

145-180

0.8941

13

0.8941

0.9538

BhUNICAMP065

JF812641

(AATA)3

55

198

ATGTCACGTTATCAGCAGAAG

GGGCCACATCACCTTTT

1

200

0

1

0

0

BhUNICAMP066

JF812641

(TCTT)3

55

218

ATGACAAACTGACCGTATC

TAGCAATTTTCTTTATCAACT

10

217-231

0.7489

9

0.7598

0.8923

BhUNICAMP067

JF812642

(CT)17...(CT)5

60

301

ACCCCCTGTAATTGTTGTCC

GATTCAGATGGTTAGCGTGTTA

15

245-335

0.8961

14

0.8941

0.9354

  1. * Species evaluated: Urochloa humidicola (Rendle) Morrone & Zuloaga, Urochloa brizantha (Hochst. ex A. Rich.) R.D. Webster, Urochloa decumbens (Stapf) R.D. Webster, Urochloa dictyoneura (Figure & De Not.) Veldkamp, Urochloa ruziziensis (R. Germ. & C.M. Evrard) Crins
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BMC Research Notes

ISSN: 1756-0500

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