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Figure 1 | BMC Research Notes

Figure 1

From: Opposite temperature effect on transport activity of KCC2/KCC4 and N(K)CCs in HEK-293 cells

Figure 1

Temperature effect on KCC2 and KCC4 in HEK-293 cells. HEK-293 cells were transfected with KCC2 (a) and KCC4 (b). Before 86Rb+ uptake cells were incubated for 30 min in preincubation buffer at room temperature or 37°C. An empty vector was used for mock transfection. Both KCC2 (p = 0.004) and KCC4 (p = 3.42 × 10-5) showed significant increase in transport activity after preincubation at room temperature compared to 37°C (left handed part). Relative transport activity was calculated after background subtraction (right handed part). All 86Rb+ uptakes were sensitive to 2 mM furosemide. KCC2 transport activity could be significantly stimulated by 1 mM NEM. ***, p < 0.001; **, p < 0.005; *, p < 0.05.

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