Analysis of the primary sequence of the zebrafish RyRs. (a) Schematic representation of the protein sequence of zebrafish RyRs compared to the human isoforms. The figure shows the three human RyRs at the top and the five zebrafish RyRs below. Sequence equivalent to MIR and RIH domains identified in all five zebrafish RyRs. Three SPRY domains, four copies of the RyR repeated domain, a single copy of the RIH associated domain and a putative EF hand domain were also identified within the zebrafish RyRs. Transmembrane domains (TM 4-6) and putative pore forming regions (TM 8-10) were also present. (b) Alignment of 36 RyR orthologues to show the sequences of the pore and transmembrane regions (M8, pore, M10). Letters A and B indicate regions not shown in alignment. Bar graph indicates the number of different amino acids among the different isoforms at the indicated residues.