Phylogenetic tree of the vertebrate RyR gene family. The evolutionary relationship between the zebrafish ryr orthologues and cloned full-length ryr genes of other vertebrate species was determined. RyR protein sequences were aligned using the T-coffee multiple sequence alignment. The phylogenetic tree was generated using the equal angled method available on the SplitTree4 software. The branch length of the tree is proportional to evolutionary distance. The family of human inositol 1,4,5-trisphosphate receptors (IP3R) was used as an out-group. Three monophyletic clades corresponding to (1) RyR1, (2) RyR2 and (3) RyR3 gene subfamilies were identified. A full description of the sequences used can be found in Table 2. through to adulthood.