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Figure 5 | BMC Research Notes

Figure 5

From: Diversity, mobility, and structural and functional evolution of group II introns carrying an unusual 3' extension

Figure 5

Time-course analysis of in vitro self-splicing of B.c .I4 and B.th .I6a wild-type (WT) and mutant constructs. (A) B.c.I4 WT and B.c.I4 deleted of the entire 3' extension (B.c.I4_dS1S2) spliced in (NH4)2SO4 buffer; (B) same constructs as in (A) spliced in KCl buffer; (C) B.c.I4 deleted of the branchsite adenosine only (B.c.I4_dA) or deleted of the branchsite adenosine and the entire 3' extension (B.c.I4_dA_dS1S2) spliced in KCl buffer; and (D) B.th.I6a WT and B.th.I6a deleted of the entire 3' extension (B.th.I6a_dS1S2) spliced in KCl buffer. Splicing was performed in 40 mM MOPS (pH 7.5), 100 mM MgCl2 and either 500 mM (NH4)2SO4 (panel A) or 500 mM KCl (panels B, C, and D) at 47°C. The relative fractions of released lariat intron were computed from the intensities of the radioactive bands using a phosphorimager. The values shown represent averages with standard deviations of one replicate from two different RNA preparations

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