A linear regression plot of the standard curves against percent methylation.(A) A plot of peak-height versus percent methylation (r = 0.998). (B) A plot of area-under-the-curve versus percent methylation (r = 0.998). For this analysis, prepared standard samples with 0, 10, 20, 30, 40, 50, 60, 70, 80, 90 and 100% methylation were analyzed. All data points were plotted as mean of triplicate samples ± S.D. Due to minimal variation in triplicate measures, error bars were not visible in the plot.