Relationship between the amount of BSC DNA template from blood and LINE-1 methylation. The amount of BSC DNA was varied (left to right, 10 (red), 5 (blue), 2.0 (green), 1 (purple), 0.5 (light blue) and 0.1 ng (light green)) and HRM was used to measure LINE-1 methylation. (A) Fluorescence curves for varying amounts of DNA. Each sample was analyzed in triplicate. Note that at 40 cycles, the 0.1 ng curve did not fully reach a plateau. (B) Determination of percent methylation as a function of amount of template BSC DNA. Values are expressed as mean percent ± S.D. Data were analyzed by one-way ANOVA with Tukey's post hoc test.