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Table 2 Reproducibility of LINE-1 methylation

From: A refined, rapid and reproducible high resolution melt (HRM)-based method suitable for quantification of global LINE-1 repetitive element methylation

 

Samples

Range of

Std Dev

Root Mean Square Error

Grand

Mean

CV%

Intra-assay variation*

39

0.14-2.29

1.21

83.70%

1.44%

Inter-assay variation**

13

0.10-0.70

0.41

83.70%

0.49%

  1. *Analysis included 3 replicates for each of the 13 participants on each of 3 plates (39 samples)
  2. **Averages of 3 replicates for each of the 13 participants were compared between 3 plates (13 samples)