Table 1 List of primers used in this study
From: Occurrence of the invasion associated marker (iam) in Campylobacter jejuni isolated from cattle
Primer | Sequence (5 '-3 ') | Properties | Product size | Ref. |
|---|---|---|---|---|
16S rRNA-F 16S rRNA-R | 5'-ATCTAATGGCTTAACCATTAAAC-3 '5 '-GGACGGTAACTAGTTTAGTATT-3 ' | Campylobacter specific | 850 bp | [19] |
map A-F map A-R | 5 '-CTATTTTATTTTTGAGTGCTTGTG-3 ' 5 '-GCTTTATTTGCCATTTGTTTTATTA-3 ' | C. jejuni specific | 589 bp | [20] |
cad F-F cad F-R | 5 '-TTGAAGGTAATTTAGATATG-3 ' 5 '-CTAATACCTAAAGTTGAAAC-3 ' | cad F detection | 400 bp | [10] |
cdt B-F cdt B-R | 5 '-GTTAAAATCCCCTGCTATCAACCA-3 ' 5 '-GTTGGCACTTGGAATTTGCAAGGC-3 ' | cdt B detection | 495 bp | [22] |
cia B-F cia B-R | 5 '-TTTTTATCAGTCCTTA-3 ' 5 '-TTTCGGTATCATTAGC-3 ' | cia B detection | 986 bp | [21] |
Primer 1290 | 5 '-GTGGATGCGA-3 ' | RAPD-typing | Variable | [11] |
1.6-F 1.6-R | 5 '-GCGCAAAATATTATCACCC-3 ' 5 '-TTCACGACTACTATGCGG-3 ' | iam-specific | 518 bp | [11] |
Iam1-F Iam1-R | 5 '-AACATTAGCGAGGAAGAT-3 ' 5 '-GTATATTCTTTAAGAGGGGTAG-3 ' | iam-specific (qRT-PCR) | 160 bp | This study |
Iam2-F Iam2-R | 5 '-AACATTAGCGAGGAAGAT-3 ' 5 '-TCATTTAAACCGACCATTT-3 ' | iam-specific (qRT-PCR) | 160 bp | This study |
Iam- 81176-F Iam- 81176-R | 5 '-AAGATAGCATACAAGAACT-3 ' 5 '-ATTCACGACTACTATAAGG-3 ' | iam-81176-specific (qRT-PCR) | 160 bp | This study |