Table 1 Primer sequences used to amplify and sequence 12.6 Kb of canine PTPN22 in three atopic and three non-atopic WHWTs
PCR product | Forward amplification primer | Reverse amplification primer | Internal forward sequencing primer | Internal reverse sequencing primer | Predicted gene region | Product size |
|---|---|---|---|---|---|---|
1 | CCTCATCAGGTGCTCTTCGT | GGTTTTGCCTCTCTCCCTTC | TGAAGTGGAAGAGTCTCAGAGC | AGAAAAGGCAGAAGGCCAGT | 5'UTR, exon 1 | 1041 |
2 | GGCTCTGTCCTGAATTGGAG | TCTGCCCTTACCAGGACACT | - | - | Exons 2,3 | 858 |
3 | CCAAATAAGAGGTCGGGGTA | CTACTGGGAAAATGGGCAAA | AGAAAAGGGAAGGAAGGACA | TCTGTCCTTCCTTCCCTTTTC | Exons 4,5 | 863 |
4 | ACCACAGTTGACCTTGGATAA | AGATGAAGGCACATCATGGTC | - | ACATCAAAGGTCCCCTACTCC | Exons 6,7 | 1182 |
5 | CCACTTGAACTGGTGAAGCA | ACCAGTCCTTCCACAACCAG | - | GGATGGAACCCCATATTGAA | Exons 8 | 1172 |
6 | TGCTCTGGGAAGTAGGGATG | CAAGGCAAGGGACATAGGAA | AATCCACCACAACCAAACCT | AGCCCGTATTTCCAACTTCC | Exons 9,10 | 1267 |
7 | CCGAAATGAGGTAGGCAAAC | GCCCTGTCACTCACCCTTAT | - | - | Exons 11 | 483 |
8 | TGGAAACTCACCTCTTTTGTGA | TTCTTTGAGAAGGAAAAGGAAGAA | CAGAGTGGGAGACAAAAGCA | CCAGCTCCTTGGTGTCTCTC | Exons 12,13 | 1296 |
9 | GAAGCAGCAGAAAACCTCCTA | ACCCCACATCCTCTAGCACA | GATCCCCATTTGCATTGTTC | TGGCCCAATTCTTAGGAGTGT | Exons 14,15 | 889 |
10 | GGGTAAAGGATGCGTTTTCA | TGGGAGCTATTATGGGAACC | - | - | Exons 16 | 332 |
11 | TGAGGCTCCAGTTATGGTTCA | CAGTCTTGTTCTCAATCTGCTTC | AAGTGGGACCTAAATGGAAAAG | CCTTTTCCATTTAGGTCCCACT | Exons 17,18 | 747 |
12 | GGATGGGAAAAAGTAGCAAGG | TTCTGATACAAAGAGCCATAGCA | - | - | Exon 19 | 410 |
13 | TTCCCTTTAGTGTTGGGCTTT | TTGGCTTTGGCTAGTCACATT | - | - | Exon 20 | 92 |
14 | GGCTGAATTACCAAAGGTTGT | TTCACAAATCCATCGTCAGG | TCGCAAAATCTGACTTGTGG | GGGAGATGTGCAAGGAATTT | Exon 21, 3'UTR | 550 |