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Table 3 icaA expression values using RNA extracted from the three different kits

From: Comparison of RNA extraction methods from biofilm samples of Staphylococcus epidermidis

Extraction kit

Strain

16S rRNA Ct

icaA Ct

icaA expression

FastRNA®

1457

17.36 ± 0.41

31.48 ± 0.28

5.62 × 10-5

 

M187

12.27 ± 0.81

27.07 ± 1.05

3.48 × 10-5

PureZOL™

1457

17.99 ± 0.30

31.84 ± 0.11

6.77 × 10-5

 

M187

15.71 ± 0.83

29.58 ± 1.94

6.20 × 10-5

PureLink™

1457

15.25 ± 0.12

32.56 ± 0.11

0.62 × 10-5 **

 

M187

13.83 ± 0.67

31.13 ± 4.02

0.62 × 10-5 **

  1. Total RNA (40 ng) was used to synthesize cDNA for qPCR. The icaA expression was calculated by the delta Ct method (2ΔCt). The values represent the mean plus or minus standard deviation of 2 to 4 independent experiments (**P < 0.01).
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BMC Research Notes

ISSN: 1756-0500

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