Figure 2

Localization of Nogo-B in macrophages with different morphology. Monocyte-derived macrophages showing different morphologies and the respective Nogo-B staining pattern (left panel). Structures similar to the staining pattern are visible in phase contrast microscopy (right panel): (a) Quiescent, round cell with reticular Nogo-B staining. (b) Activated cell with elongate and mainly punctate Nogo-B positive areas in the periphery (arrows, see inlay). (c) Radial, elongate Nogo-B staining patterns in the cell periphery of an elongated cell (arrows, see inlay). Note the unstructured Nogo-B accumulation in the left area of the cell. (d) Polarized, longish Nogo-B structures in an elongated cell (arrows, see inlay) (e) Strong Nogo-B translocation to the trailing end of migrating macrophages (arrow) with remnant reticular staining. All representative cell images are of one donor, showing comparable staining patterns observed in independent cultures of six donors. All images were acquired by conventional fluorescence microscopy using a DMI 4000B microscope and Application Suite V3.1 by Leica and represent cells cultured in 24-well cell culture plates.