Confocal imaging of Nogo-B and the tubulin network, endoplasmic reticulum and Golgi apparatus. (a) Shows the strong association of Nogo-B with the tubulin network. Note the Nogo-B accumulation and detachment from tubulin in the cell protrusion. (b) Nogo-B co-localizes with the endoplasmic reticulum in the cell center, whereas no enriched calnexin signal can be observed in peripheral areas of Nogo-B accumulation. (c) Nogo-B shows a low presence in the Golgi apparatus compared to surrounding structures. All images are representative of regularly observed staining patterns/co-localizations in independent macrophage cultures of six different donors. Cells were analyzed by confocal laser scanning microscopy using an UltraVIEW RS (Perkin Elmer) mounted on an Olympus IX-70 inverse microscope. Images were acquired using the UltraVIEW RS software (Perkin Elmer). Shown are z-stacks of 0.5 μm thickness.