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Table 1 WGA using three different commercial kits on DNA extracted from microdissected tissue and from blood

From: Laser capture microdissection (LCM) and whole genome amplification (WGA) of DNA from normal breast tissue --- optimization for genome wide array analyses

   

DNA SOURCE

   
   

LCM tissue

 

Blood

 
 

Recommended input (μg)

Expected output (μg)

Input (μg)

Output (μg)

Input (μg)

Output (μg)

Repli-g (Qiagen) ®

>0.010

Approx. 10

0.015-0.030

2.0 (0.5-5.1)

0.013

5.2 (3.9-6.5)

GenomiPhi (GE Healthcare)

>0.010

4-7

0.013-0.050

2.0 (1.1-3.6)

0.013

4.5 (4.2-4.8)

BioScore™ (Enzo Life Sciences)

0.100

>10

0.040-0.100

12.9 (11.9-14.3)

0.100

11.8 (10.3-13.3)

  1. The recommended input and expected output are noted for each kit as well as the results from this experiment. The output from this experiment is given as mean values and the range within brackets.