Growth curves for duplicate fermentor cultures of S. coelicolor A2(3) M145 grown in N, P and C-limited Modified Evans Medium. Three dry weights per sample per time point were averaged to provide each datum. To align the exponential phases of the cultures with the N1 culture the time points for the N2, C1 and C2 cultures have been shifted by -12, -8 and -8 h respectively. The [NH4+] assay data (where available) for the N-limited cultures are also shown. The N5-7 time points, aligned to the N1 growth curve, are indicated by dashed red bars.