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Figure 1 | BMC Research Notes

Figure 1

From: Comparative nucleic acid transfection efficacy in primary hepatocytes for gene silencing and functional studies

Figure 1

Effect of DNA:lipid ratio and DNA concentration on transfection efficiency. Cells were transfected with pRNAT-H1.1/neo GFP plasmid complexed with Metafectene (Met), Metafectene Pro (Met Pro), Lipofectamine 2000 (Lipo 2000) or Targefect (Targe), for 16 hr. (A) DNA:lipid ratio. Two μg DNA was complexed with lipid at ratios of 1:0.5, 1:1, 1:2, 1:4, 1:8, 1:16. The percentage of GFP-positive cells was determined 24 hr after transfection. Values represent the average of 4 independent experiments. (B) DNA concentration. Using a 1:4 DNA:lipid ratio, cells were transfected with 1, 2, 3, 4, and 5 μg of DNA. The percentage of GFP-positive cells was determined 24 hr after transfection. Values represent the average of 3 independent experiments.

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BMC Research Notes

ISSN: 1756-0500

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