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Figure 2 | BMC Research Notes

Figure 2

From: Comparative nucleic acid transfection efficacy in primary hepatocytes for gene silencing and functional studies

Figure 2

Influence of culture medium and incubation time on transfection efficiency. Primary hepatocytes were transfected with 2 μg of plasmid and Metafectene Pro (Met Pro), Lipofectamine 2000 (Lipo 2000) and Targefect (Targe) at a ratio of 1:4. Cells were incubated with DMEM or Opti-MEM for 3 hr or 16 hr. The percentage of GFP-positive cells was determined 24 hr after transfection. (A) GFP images were taken from cells incubated with DMEM media. (B) Quantification of GFP-positive cells after 3 hr transfection in the presence of DMEM or OptiMEM with or without Virofect enhancer. (C) Quantification of GFP-positive cells after 16 hr transfection in the presence of DMEM or OptiMEM with or without Virofect enhancer. Values represent the average of 5 independent experiments. *p < 0.01, **p < 0.05 between DMEM and Opti-MEM, and DMEM/Viro and Opti-MEM/Viro.

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