Figure 3From: Comparative nucleic acid transfection efficacy in primary hepatocytes for gene silencing and functional studiesGene silencing using plasmid transfection. Primary hepatocytes cultured in DMEM were transfected with plasmids (2 μg) expressing shRNA to knock-down SREBP1 or FABP5 and Metafectene Pro (1:4 ratio), for 16 hr. (A) SREBP-1 gene silencing; (B) FABP5 gene silencing. The level of mRNA was quantified by real-time RT-PCR (A and B, top). The level of silencing is expressed relative to medium-treated cells. Proteins were quantified by Western blot (A and B, bottom). β-actin was used as a normalizer. Data are representative of two independent experiments (n = 3); *p < 0.05; **p < 0.01 shSCR compared to shSREBP-1 or shFABP5.Back to article page