Skip to main content
Figure 3 | BMC Research Notes

Figure 3

From: Comparative nucleic acid transfection efficacy in primary hepatocytes for gene silencing and functional studies

Figure 3

Gene silencing using plasmid transfection. Primary hepatocytes cultured in DMEM were transfected with plasmids (2 μg) expressing shRNA to knock-down SREBP1 or FABP5 and Metafectene Pro (1:4 ratio), for 16 hr. (A) SREBP-1 gene silencing; (B) FABP5 gene silencing. The level of mRNA was quantified by real-time RT-PCR (A and B, top). The level of silencing is expressed relative to medium-treated cells. Proteins were quantified by Western blot (A and B, bottom). β-actin was used as a normalizer. Data are representative of two independent experiments (n = 3); *p < 0.05; **p < 0.01 shSCR compared to shSREBP-1 or shFABP5.

Back to article page