Figure 4
miRNA and siRNA transfection. (A) miRNA transfection. Primary hepatocytes were cultured in 6-cm dishes at 1 × 106 cell/dish and transfected with 1.5, 3.0 and 4.5 μg Dy547-labeled miRIDIAN microRNA Mimic Transfection Control. The percentage of positive cells was determined 24 hr after transfection. (B) siRNA transfection to knock-down FABP5. Cells were cultured in 6-well plates and incubated with 2 μg FABP5 siRNA, FABP1 siRNA or non-targeting siRNA (ratio 1:3). Cells were harvested 72 or 96 hours later. FABP5 levels were analyzed by Western blot. FABP1 is a member of the FABP family. Only the siRNA that is specific for FABP5 reduces its protein levels. siNT, non-targeting siRNA; siF1, FABP1 siRNA; siF5, FABP5 siRNA.