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Table 1 Primer pair sequences and allele distribution of 10 microsatellites among clone A, clone D and clone A+ of gibel carp*

From: A novel nucleo-cytoplasmic hybrid clone formed via androgenesis in polyploid gibel carp

Locus (YJ) Primer pair sequence Annealing tempreture Allele size (bp)
    Clone A Clone D Clone A+
0001 5'-CTGGCATGAAGACTGGCTC-3'
5'-CAACAACACATATCAGCTCC-3'
53°C 84, 94, 100 88, 92, 96 84, 94, 100
0003 5'-TGAAGTTATTAGAAAGAGAG-3'
5'-CTTGATGATGTCTATGTGTG-3'
53°C 270, 288, 302 260, 290, 292 270, 288, 302
0004 5'-CATAGAGGCGTTTCATAGAG-3'
5'-CAGATAAATACAGTAAGCCA-3'
50 - 55°C 210, 220, 224 218, 222, 226 210, 220, 224
0005 5'-TAATAAGGTACATAGTCATAG-3'
5'-GTCAGCCTCCACCACGAATC-3'
50 - 55°C 226, 230, 234 222 226, 230, 234
0033 5'-CGGACACAAGAACGCCAAC-3'
5'-GGACTGGGCTGAAACTGATG-3'
50 - 55°C 172, 178 176 172, 178
0039 5'-GAAGAATACTTTATGACTGAGG-3'
5'-GACCAAGACAGACAGCCCAG-3'
50 - 55°C 138, 150, 156 136, 138, 156 138, 150, 156
0042 5'-GGCCACCTACAGTATATGC-3'
5'-GAAAACCAGGACCGACATG-3'
50 - 55°C 110, 114, 116 110, 112, 118 110, 114, 116
0040 5'-CCAGTATTAGGGAGCGTTC-3'
5'-GTTTCGTCTTCACAATCAGAA-3'
50 - 55°C 124, 138 132, 148 124, 138
0010 5'-GATGGTTGTGCTGTGAGCT-3'
5'-GAGTTCGTTTACATCTGGAC-3'
53°C 150, 156, 166 152, 160, 162 150, 156, 166
0022 5'-CACCAACTTTAGGCACATTTG-3'
5'-CCAGACTCCCACGTCATG-3'
53°C 144, 156 140, 162, 170 144, 156
Total 10   27 25 27
  1. *For each clone and each generation, 10 individuals were sampled and analyzed, and clone A+ samples of three generations, such as the third, fifth and seventh generation, were examined. No any individual difference was detected within each clone and among different generations.