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Table 1 Primer pair sequences and allele distribution of 10 microsatellites among clone A, clone D and clone A+ of gibel carp*

From: A novel nucleo-cytoplasmic hybrid clone formed via androgenesis in polyploid gibel carp

Locus (YJ)

Primer pair sequence

Annealing tempreture

Allele size (bp)

   

Clone A

Clone D

Clone A+

0001

5'-CTGGCATGAAGACTGGCTC-3'

5'-CAACAACACATATCAGCTCC-3'

53°C

84, 94, 100

88, 92, 96

84, 94, 100

0003

5'-TGAAGTTATTAGAAAGAGAG-3'

5'-CTTGATGATGTCTATGTGTG-3'

53°C

270, 288, 302

260, 290, 292

270, 288, 302

0004

5'-CATAGAGGCGTTTCATAGAG-3'

5'-CAGATAAATACAGTAAGCCA-3'

50 - 55°C

210, 220, 224

218, 222, 226

210, 220, 224

0005

5'-TAATAAGGTACATAGTCATAG-3'

5'-GTCAGCCTCCACCACGAATC-3'

50 - 55°C

226, 230, 234

222

226, 230, 234

0033

5'-CGGACACAAGAACGCCAAC-3'

5'-GGACTGGGCTGAAACTGATG-3'

50 - 55°C

172, 178

176

172, 178

0039

5'-GAAGAATACTTTATGACTGAGG-3'

5'-GACCAAGACAGACAGCCCAG-3'

50 - 55°C

138, 150, 156

136, 138, 156

138, 150, 156

0042

5'-GGCCACCTACAGTATATGC-3'

5'-GAAAACCAGGACCGACATG-3'

50 - 55°C

110, 114, 116

110, 112, 118

110, 114, 116

0040

5'-CCAGTATTAGGGAGCGTTC-3'

5'-GTTTCGTCTTCACAATCAGAA-3'

50 - 55°C

124, 138

132, 148

124, 138

0010

5'-GATGGTTGTGCTGTGAGCT-3'

5'-GAGTTCGTTTACATCTGGAC-3'

53°C

150, 156, 166

152, 160, 162

150, 156, 166

0022

5'-CACCAACTTTAGGCACATTTG-3'

5'-CCAGACTCCCACGTCATG-3'

53°C

144, 156

140, 162, 170

144, 156

Total

10

 

27

25

27

  1. *For each clone and each generation, 10 individuals were sampled and analyzed, and clone A+ samples of three generations, such as the third, fifth and seventh generation, were examined. No any individual difference was detected within each clone and among different generations.