A novel nucleo-cytoplasmic hybrid clone formed via androgenesis in polyploid gibel carp

BMC Research Notes

Table 1 Primer pair sequences and allele distribution of 10 microsatellites among clone A, clone D and clone A⁺ of gibel carp*

Locus (YJ)	Primer pair sequence	Annealing tempreture	Allele size (bp)
			Clone A	Clone D	Clone A⁺
0001	5'-CTGGCATGAAGACTGGCTC-3' 5'-CAACAACACATATCAGCTCC-3'	53°C	84, 94, 100	88, 92, 96	84, 94, 100
0003	5'-TGAAGTTATTAGAAAGAGAG-3' 5'-CTTGATGATGTCTATGTGTG-3'	53°C	270, 288, 302	260, 290, 292	270, 288, 302
0004	5'-CATAGAGGCGTTTCATAGAG-3' 5'-CAGATAAATACAGTAAGCCA-3'	50 - 55°C	210, 220, 224	218, 222, 226	210, 220, 224
0005	5'-TAATAAGGTACATAGTCATAG-3' 5'-GTCAGCCTCCACCACGAATC-3'	50 - 55°C	226, 230, 234	222	226, 230, 234
0033	5'-CGGACACAAGAACGCCAAC-3' 5'-GGACTGGGCTGAAACTGATG-3'	50 - 55°C	172, 178	176	172, 178
0039	5'-GAAGAATACTTTATGACTGAGG-3' 5'-GACCAAGACAGACAGCCCAG-3'	50 - 55°C	138, 150, 156	136, 138, 156	138, 150, 156
0042	5'-GGCCACCTACAGTATATGC-3' 5'-GAAAACCAGGACCGACATG-3'	50 - 55°C	110, 114, 116	110, 112, 118	110, 114, 116
0040	5'-CCAGTATTAGGGAGCGTTC-3' 5'-GTTTCGTCTTCACAATCAGAA-3'	50 - 55°C	124, 138	132, 148	124, 138
0010	5'-GATGGTTGTGCTGTGAGCT-3' 5'-GAGTTCGTTTACATCTGGAC-3'	53°C	150, 156, 166	152, 160, 162	150, 156, 166
0022	5'-CACCAACTTTAGGCACATTTG-3' 5'-CCAGACTCCCACGTCATG-3'	53°C	144, 156	140, 162, 170	144, 156
Total	10		27	25	27

*For each clone and each generation, 10 individuals were sampled and analyzed, and clone A⁺ samples of three generations, such as the third, fifth and seventh generation, were examined. No any individual difference was detected within each clone and among different generations.

ISSN: 1756-0500