RpS3 gene dissociation curves in O. nubilalis (A) and D. melanogaster (B). (A) Dissociation curves of the qPCR RpS3 gene amplification products from eight O. nubilalis DNA samples (0.1 and 0.01 ng/μl of qPCR reaction). Each DNA sample was obtained from individual thoraxes from insects from the PH, FR or M colonies. Five melting temperature peaks were obtained at 75.75°C, 75.67°C, 75.60°C, 75.52°C and 75.45°C. (B) Dissociation curves of the qPCR products from 7 D. melanogaster DNA pooled samples (0.5 and 0.1 ng/μl of qPCR reaction) from thoraxes of 4 adults from the Or-R strain. A single melting temperature peak was obtained at 74,03°C.