Insect

Target gene

Primer pair

Primer name

Sequence (5'→3')


D. melanogaster

RpS3

s31

s31 F

TCTTTCTTTTCTGCGCACCA

  
s31 R

TCGCATTCATTTTGACGTCG


BarH1

h14

h14 F

CCAGGACGATCCGTTGACA

  
h14 R

GATCTGATCCTCGTCGTCCG

 
h15

h15 F

TCCAGGTGTGAGCGGTACG

  
h15 R

ATTAGCACACGCACACAATCG


BarH2

h24

h24 F

CAGAACCAAATGGAAGCGTCA

  
h24 R

TCGGCCAGCAGTTCCAAG

 
h27

h27 F

GGAGGAACTGGCCCTGGA

  
h27 R

GGAGGGTTGAATTCTCTCGGA

O. nubilalis

RpS3

s31

s31 F

GAGCTACTGGGAGAGAAGG

  
s31 R

GATGTTGAAACGCTTCTGGA

 
s32

s32 F

GCGTTTCAACATCCCTGAACA

  
s32 R

GGCGACTTTCTCAGCGTACAG


tpi

t1

t1 F

GGCGACAAGAATCAAATCAATG

  
t1 R

AGGACCCTTTTTCAGAGTGTTCAC


ldh

l1

l1 F

GAATAAATCGGGCTCGAAGGAC

  
l1 R

TCACGCGAGACAGCTTCAAA


cadherin

e26

e26F

ACGGCAACAACGAGGGTCT

  
e26R

GAGATGACGTTGCGCGACT

 
e28

e28F

CGAGCCACACAGAAGACGAC

  
e28R

TGCTCGCACGGTCTATGATG

 The letters F or R at the end of the primer names refer to their respective orientation (F = forward; R = reverse). The qPCR product sizes were 51 base pairs in all reactions except for primer pairs s31 and e26 of O. nubilalis, with lengths of 70 and 52 base pairs respectively.