Denaturing gel electrophoresis of RNA isolated from leaf tissues of rheum (A), and arnebia (B) using different RNA isolation methods viz. RNeasy®, TRIzol®, Guanidine-HCl, and IHBT protocol. RNA yield (μg/100 mg tissue) is mentioned above each panel, whereas A260/280 ratio is mentioned at the bottom of the panel. To ease comparison, equal volume of RNA (2 μl) was loaded. The starting material and the volume of the DEPC-treated water used to dissolve RNA were kept same in all the four procedures. N.D., not detectable.