Ta ZYP1 shares sequence similarities withother plant species and is located on chromosome group 2 in wheat. (A) Peptides identified from the MS/MS data of the digested heterologously-expressed Ta ZYP1 protein that were identical to those within the rice Os ZYP1 sequence [TIGR rice locus identifier: LOC_Os04g37960] are highlighted in the amino acid sequence (red font). (B) Membranes prepared with DNA from nullisomic (N)-tetrasomic (T) wheat lines of Chinese Spring (CS) were hybridised with a TaZYP1-specific probe showing that TaZYP1 is located on chromosome group 2 with a copy on the A, B and D genomes (indicated by black arrowheads). The faint signal in lane 4 (N1D-T1A) was caused by uneven loading. (C) The evolutionary history of Ta ZYP1 and its homologues was inferred using the Neighbor-Joining method. The bootstrap consensus tree inferred from 10000 replicates is taken to represent the evolutionary history of the 11 taxa analysed. Branches corresponding to partitions reproduced in less than 50% bootstrap replicates are collapsed. The percentages of replicate trees in which the associated taxa clustered together in the bootstrap test (10000 replicates) are shown next to the branches. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. All positions containing alignment gaps and missing data were eliminated only in pairwise sequence comparisons. There were a total of 1019 positions in the final dataset. Phylogenetic analysis was conducted using MEGA4. Ta-Triticum aestivum; Bd-Brachypodium distachyon; Os-Oryza sativa; Zm-Zea mays; Vv-Vitus vinifera; Rc-Ricinus communis; Bo-Brassica oleracea; At-a-Arabidopsis thaliana ZIP1a; At-b-Arabidopsis thaliana ZIP1b; Mm-Mus musculus; Sc-Saccharomyces cereviseae.