Localisation of Ta ZYP1 and Ta ASY1 across four Taasy1 RNAi knock-down mutants. In general, Ta ZYP1 (green) localisation within the Taasy1 mutants was similar to that of wild-type with lengthening of the Ta ZYP1 signal observed as diffuse disrupted tracts during pachytene. Compared to wild-type, TaASY1 (red) signals were diffuse and slightly weaker in the mutants and persisted at higher levels through to diplotene. Chromatin (blue) was counter-stained with DAPI. Scale bar, 7.5 μm.