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Figure 5 | BMC Research Notes

Figure 5

From: Evaluation of suitable reference genes for gene expression studies in porcine alveolar macrophages in response to LPS and LTA

Figure 5

Determination of the optimal number of reference genes for normalization. The geNorm software calculates the normalization factor from an increasing number of genes (starting with at least two) for which the variable V defines the pairwise variation between two sequential normalization factors. The lower the pairwise variation, the better is the combination of genes for reference. V5/6 for example, shows the variation between the normalization factors of five genes in relation to six genes and shows that six genes is the combination providing the lowest pairwise variation. Irrespective to stimulation: when all the stimulated and non-stimulated control were considered together; Control: no stimulation; LPS: lipopolysaccharide; LTA: lipoteichoic acid; LPS + LTA (combined): lipopolysaccharide used together with lipoteichoic acid.

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