Skip to main content

Table 1 Selected candidate reference genes, primers, and PCR reactions efficiencies

From: Evaluation of suitable reference genes for gene expression studies in porcine alveolar macrophages in response to LPS and LTA

Gene name

GenBank accession number

Primer sequence

Amplicon length (bp)

Amplification efficiency (%)

aR2

Average Ct of cDNA

      

Control

LPS

LTA

Combined

B2M

NM_213978.1

F:ACTTTTCACACCGCTCCAGT

R:CGGATGGAACCCAGATACAT

180

89.45

0.992

25.46

24.30

23.58

23.34

BLM

NM_001123084.1

F:TCCTCACCTTCTGCATTTCC

R:GTGGTGGCTGAGAATCCTGT

152

93.12

0.993

30.47

28.58

27.54

28.06

GAPDH

AF017079.1

F:ACCCAGAAGACTGTGGATGG R:ACGCCTGCTTCACCACCTTC

247

89.45

0.994

36.96

35.59

33.92

34.32

HPRT1

NM_001032376.2

F:AACCTTGCTTTCCTTGGTCA

R:TCAAGGGCATAGCCTACCAC

150

91.88

0.997

29.21

28.16

28.50

27.89

PPIA

NM_214353.1

F:CACAAACGGTTCCCAGTTTT

R:TGTCCACAGTCAGCAATGGT

171

91.32

0.997

23.79

22.88

23.38

23.27

RPL4

DQ845176.1

F:AGGAGGCTGTTCTGCTTCTG

R:TCCAGGGATGTTTCTGAAGG

185

90.21

0.993

25.47

24.92

23.57

24.19

SDHA

DQ178128.1

F:AGAGCCTCAAGTTCGGGAAG

R:CAGGAGATCCAAGGCAAAAT

149

92.24

0.996

30.35

29.28

28.21

28.11

TBP

DQ178129.1

F:ACGTTCGGTTTAGGTTGCAG

R:GCAGCACAGTACGAGCAACT

118

99.43

0.997

31.81

30.59

31.11

30.25

YWHAZ

DQ178130.1

F:ATTGGGTCTGGCCCTTAACT

R:GCGTGCTGTCTTTGTATGACTC

146

94.52

0.994

24.50

23.74

23.78

23.23

  1. a R2, correlation coefficient calculated from slope of the standard curve