Evaluation of suitable reference genes for gene expression studies in porcine alveolar macrophages in response to LPS and LTA

Table 2 Expression stability of nine candidate reference genes evaluated by BestKeeper software

	B2M	BLM	GAPDH	HPRT1	PPIA	RPL4	SDHA	TBP	YWHAZ	BI
Irrespective to stimulation
n	48	48	48	48	48	48	48	48	48	48
SD [± Ct]	1.05	1.10	1.61	0.78	0.79	0.89	1.03	0.81	0.76	0.83
CV [% Ct]	4.36	3.84	4.57	2.73	3.37	3.64	3.57	2.63	3.20	3.04
Control
n	12	12	12	12	12	12	12	12	12	12
SD [± Ct]	0.99	1.33	1.07	0.93	0.63	1.07	1.00	0.74	0.82	0.93
CV [% Ct]	3.88	4.35	2.91	3.19	2.65	4.18	3.30	2.34	3.34	3.27
LPS
n	12	12	12	12	12	12	12	12	12	12
SD [± Ct]	0.46	0.73	1.25	0.61	0.60	0.75	0.44	0.73	0.56	0.59
CV [% Ct]	1.89	3.52	2.54	2.16	2.60	3.00	1.52	2.39	2.35	2.16
LTA
n	12	12	12	12	12	12	12	12	12	12
SD [± Ct]	0.95	0.42	1.53	0.61	0.75	0.73	0.91	0.70	0.72	0.74
CV [% Ct]	4.04	4.51	1.53	2.15	3.19	3.11	3.23	2.27	3.04	2.75
LTA + LPS
n	12	12	12	12	12	12	12	12	12	12
SD [± Ct]	0.59	0.39	1.33	0.46	0.98	0.36	0.39	0.49	0.53	0.34
CV [% Ct]	2.52	1.37	3.89	1.66	4.21	1.49	1.38	1.62	2.30	1.26

Descriptive statistics of nine candidate reference genes based on their cycle threshold (Ct) values. In the last column the BestKeeper (BI) index is computed together with the same descriptive parameters for nine genes. Abbreviations: n number of observations, CV [%Ct] the coefficient of variance expressed as a percentage on the Ct level, SD [± Ct] the standard deviation of the Ct. Results from overall tissues irrespective of stimulation, non-stimulated control and different stimulations (LPS, LTA and LPS + LTA) are shown

ISSN: 1756-0500