Table 2 Primer used for analysis of site-specific ChIP products

Sp1 binding site a

Sp1-a

For: 5'-GCACAGATTTGGCTCGACCTGGA-3'

Rev: 5'-GAGCGGGTGCCCTGAGGAGTTAATT-3'

Sp1 binding site b

Sp1-b

For: 5'-TGGCCTTGGGTCCCCGCT-3'

Rev: 5'- AGGGCGGGAGGAGGAGGGAC-3

Sp1 binding site c

Sp1-c

For: 5'-TAGACGTCCGGGCAGCCCCC-3'

Rev: 5'-TCGGGACTCCGGCCGCCT-3'

Sp1 binding site d

Sp1-d

For: 5'-AGACCGGACGACAGGCCACCT-3'

Rev: 5'-TCCCGATCAATACTGGACGGAGTCAG-3'

RsaI cutting site

RsaI

For: 5'-TGCCATTATCCGACGCTGGCTCTA-3'

Rev: 5'-GAGCGGGTGCCCTGAGGAGTTAATT-3'

BfaI cutting site

BfaI

For: 5'-GCACAGATTTGGCTCGACCTGGA-3'

Rev: 5'-AGGGCGGGAGGAGGAGGGAC-3'

BfaI 2nd cutting site

BfaI 2

For: 5'-TGGCCTTGGGTCCCCGCT-3'

Rev: 5'-CTTGGGTCCCCGCTGCTGGTT-3'

BfaI 3rd cutting site

BfaI 3

For: 5'-TAGACGTCCGGGCAGCCCCC-3'

Rev: 5'-GGTGGCCTGTCGTCCGGTCTG-3'

SacI cutting site

SacI

For: 5'-AGACCGGACGACAGGCCACCT-3'

Rev: 5'-CTTTTCCTCCAGAGCCCGACTCG-3'

ChIP control

GAPDH unrelated region

GAPDH

For: 5'-GAGGAAGAGAGAGACCCTCACTG-3'

Rev: 5'-AGGGGTCTACATGGCAACTG-3'