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Figure 2 | BMC Research Notes

Figure 2

From: Selection of reference genes in different myocardial regions of an in vivo ischemia/reperfusion rat model for normalization of antioxidant gene expression

Figure 2

Pairwise variation of candidate reference genes. Pairwise variation (Vn/n+1) was analyzed between the normalization factors NF(n) and NF(n + 1) by geNorm software to determine the optimal number of reference genes required for RT-qPCR data normalization the Right Ventricle (RV) (n = 20), Left Remote Region (RR) (n = 20) and Ischemic/Reperfused Region (IRR) (n = 20). In the RV, V2/3 is 0.135, and the two genes hmbs and hprt are sufficient for normalizing gene expression data. In the RR, analysis of pairwise variation shows that three reference genes should be included for gene expression studies in order to obtain a value below 0.15 (V3/4 = 0.137). Reference genes in the RR therefore should be hmbs, tbp, hprt. Finally, in the IRR, analysis of pairwise variation shows that six reference genes should be included for gene expression studies in order to obtain a value below 0.15 (V6/7 = 0.148). Reference genes in the IRR should therefore be ywhaz, pabp, hmbs, tbp, hprt, and actb.

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