Skip to main content

Table 1 Primer sequences of target genes and candidate reference genes for normalization

From: Selection of reference genes in different myocardial regions of an in vivo ischemia/reperfusion rat model for normalization of antioxidant gene expression

Gene symbol

Gene name

Accession number

Reference

Forward primer (5'-3')

Reverse primer (5'-3')

Amplic on length

PCR efficiency(%)

Tm (°C)

actb

Actin, beta

V01217

[49]

AAGTCCCTCACCCTCCCAAAAG

AAGCAATGCTGTCACCTTCCC

97

106

82.9°C

ywhaz

tyrosine 3-monooxygenase/tryptophan 5- monooxygenase activation protein zeta polypetide

NM_013011.2

[49]

GATGAAGCCATTGCTGAACTTG

GTCTCCTTGGGTATCCGATGTC

117

94

77.6°C

rpl13a

Ribosomal protein L13A

NM_173340

[49]

GGATCCCTCCACCCTATGACA

CTGGTACTTCCACCCGACCTC

132

104

83.5°C

gapdh

glyceraldehyde-3-phosphate dehydrogenase

NM_01708

 

CTACCCACGGCAAGTTCAAC

CCAGTAGACTCCACGACATAC

138

102

57°C

gusb

Glucuronidase, beta

NM_017015

 

TCACCATCGCCATCAACAACAC

GCTTATGTCCTGGACGAAGTAACC

92

94, 9

59°C

hprt

Hypoxantine guanine phosphoribosyl transferase

NM_012583

 

CCCAGCGTCGTGATTAGTGATG

TTCAGTCCTGTCCATAATCAGTCC

110

104

59°C

tbp

TATA box binding protein

NM_001004198

 

CACCGTGAATCTTGGCTGTAAAC

CGCAGTTGTTCGTGGCTCTC

124

104

58°C

hmbs

Hydroxymethylbilane synthase

NM_013168

[50]

TCTAGATGGCTCAGATAGCATGCA

TGGACCATCTTCTTGCTGAACA

76

95, 8

60°C

Pabpn1

poly(A) binding protein, nuclear 1

116697

http://medgen.ugent.be/rtprimerdb

TATGGTGCGACAGCAGAAGA

TATGCAAACCCTTTGGGATG

110

95

60°C

MnSod

Manganese Superoxide dismutase

NM_017051.2

 

ATCTGAACGTCACCGAGGAG

TAGGGCTCAGGTTTGTCCAG

141

96

59°C

Cu.ZnSod

Copper-Zinc Superoxide dismutase

NM_017050

http://medgen.ugent.be/rtprimerdb

CGAGCATGGGTTCCATGTC

CTGGACCGCCATGTTTCTTAG

101

96

50°C

txnr1

Thioredoxin reductase 1

NM_031614.2

 

GGTGAACACATGGAAGAGCA

GGACTTAGCGGTCACCTTGA

111

98

60°C

  1. Reference and antioxidant-coding gene primer sequences, original references, amplicon sizes, amplification efficiency values and accession number for the PCR analyses in the present study