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Figure 3 | BMC Research Notes

Figure 3

From: Expression and purification of hepatitis B surface antigen S from Escherichia coli; a new simple method

Figure 3

Sub-cloning of the HBV S gene and 3'-UTR in the expression vector pRP265. (A) An image of an agarose gel showing the supercoiled pRP265 and the * indicates the position of the plasmid. (B) An image of an agarose gel showing the KpnI/EcoRI digestion products of pRP265 and the * indicates the position of the linearized plasmid. (C) An image of an agarose gel showing the supercoiled pRP-HBsAg(S)-GST and the * indicates the position of the plasmid. (D) An image of an agarose gel showing the KpnI/EcoRI digestion products of pRP-HBsAg(S)-GST. The * indicates the position of the linearized plasmid and the ** indicates the position of the dropped insert. The left lane in each gel contains 1 kb DNA ladder (Promega, USA); the sizes of the respective bands is indicated on the left side of each panel.

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