Figure 3

Sub-cloning of the HBV S gene and 3'-UTR in the expression vector pRP265. (A) An image of an agarose gel showing the supercoiled pRP265 and the * indicates the position of the plasmid. (B) An image of an agarose gel showing the KpnI/EcoRI digestion products of pRP265 and the * indicates the position of the linearized plasmid. (C) An image of an agarose gel showing the supercoiled pRP-HBsAg(S)-GST and the * indicates the position of the plasmid. (D) An image of an agarose gel showing the KpnI/EcoRI digestion products of pRP-HBsAg(S)-GST. The * indicates the position of the linearized plasmid and the ** indicates the position of the dropped insert. The left lane in each gel contains 1 kb DNA ladder (Promega, USA); the sizes of the respective bands is indicated on the left side of each panel.