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Table 1 Description, primer sequence and amplicon characteristics for the five candidate reference genes tested

From: Validation of reference genes for expression analysis in the salivary gland and the intestine of Rhodnius prolixus (Hemiptera, Reduviidae) under different experimental conditions by quantitative real-time PCR

Symbol

Name

Function

Primer Sequences

Amplicon size (bp)

E (%) *

R2 **

ACT

β-actin

Involved in cell motility, structure and integrity

For 5' AATCAAGATCATTGCTCCACCAG 3' Rev 5' TTAGAAGCATTTGCGGTGGAC 3'

151

92%

0.996

GAPDH

Glyceralde hyde-3- phosphate dehydrogen ase

Glycolysis

For 5' GATGGCGCCCAGTACATAGT 3'

Rev 5' AGCTGACGGGGCTGTTATTA 3'

111

96%

0.9906

TUB

α-tubulin

Cytoskeleton structural protein

For 5' TTTCCTCGATCACTGCTTCC 3'

Rev 5' CGGAAATAACTGGGGCATAA 3'

129

90.1%

0.9978

L26

60S ribosomal protein

Structural constituent of ribosome

For 5' AGGTGGACAAAGATCGCAAG 3'

Rev 5' AAGTGTCCATTGCTGTCGTG 3'

117

93%

0.9991

18S

18S ribosomal RNA

Cytosolic small ribosomal subunit

For 5' TCCTTCGTGCTAGGAATTGG 3'

Rev 5' GTACAAAGGGCAGGGACGTA 3'

105

100%

0.9617

  1. *Real-time qPCR efficiency (calculated by the standard curve method)
  2. ** Regression coefficient calculated from the regression line of the standard curve