Table 3 Descriptive statistics of the five candidate reference genes based on their cyclethreshold value (CT) as calculated by the BestKeeper algorithm
Ranking→ | 1 | 2 | 3 | 4 | 5 | ||
|---|---|---|---|---|---|---|---|
R. prolixus crop | Gene name | 18S | TUB | L26 | GAPDH | ACT | |
Samples after blood feeding | Geo mean (CT) | 12.66 | 19.45 | 24.38 | 21.68 | 18.28 | |
Min-max (CT) | 12.25-13.10 | 19.18-20.27 | 23.51-24.86 | 21.23-22.43 | 18.88-20.0 | ||
Std dev (± CT) | 0.17 | 0.23 | 0.41 | 0.43 | 0.52 | ||
Std dev (± x-fold) | 1.12 | 1.18 | 1.33 | 1.35 | 1.43 | ||
Samples after T. rangeli infection | Gene name | ACT | TUB | 18S | L26 | GAPDH | |
Geo mean (CT) | 16.93 | 17.70 | 12.06 | 23.73 | 20.53 | ||
Min-max (CT) | 16.64-17.11 | 17.38-18.14 | 11.78-12.48 | 23.1-24.67 | 19.88-21.24 | ||
Std dev (± CT) | 0.12 | 0.15 | 0.17 | 0.40 | 0.41 | ||
Std dev (± x-fold) | 1.09 | 1.11 | 1.12 | 1.32 | 1.33 | ||
Samples after T. cruzi infection | Gene name | 18S | TUB | ACT | GAPDH | L26 | |
Geo mean (CT) | 12.21 | 18.02 | 16.66 | 19.22 | 23.33 | ||
Min-max (CT) | 11.91-12.44 | 17.86-18.35 | 16.39-17.30 | 18.76-19.72 | 21.99-25.42 | ||
Std dev (± CT) | 0.09 | 0.17 | 0.25 | 0.26 | 0.93 | ||
Std dev (± x-fold) | 1.07 | 1.13 | 1.19 | 1.20 | 1.90 | ||
R. prolixus | |||||||
Salivary glands | Samples after blood feeding | Gene name | 18S | ACT | TUB | GAPDH | L26 |
Geo mean (CT) | 12.03 | 19.09 | 20.00 | 22.18 | 23.05 | ||
Min-max (CT) | 11.75-12.34 | 18.49-19.89 | 19.19-20.94 | 21.21-23.48 | 21.00-24.87 | ||
Std dev (± CT) | 0.23 | 0.46 | 0.47 | 0.65 | 1.17 | ||
Std dev (± x-fold) | 1.17 | 1.38 | 1.39 | 1.56 | 2.24 | ||
Samples after T. rangeli infection | Gene name | 18S | ACT | GAPDH | TUB | L26 | |
Geo mean (CT) | 11.47 | 18.68 | 20.52 | 18.45 | 22.94 | ||
Min-max (CT) | 11.31-11.69 | 18.42-18.97 | 20.02-21.53 | 17.64-19.33 | 21.47-23.65 | ||
Std dev (± CT) | 0.11 | 0.19 | 0.26 | 0.39 | 0.49 | ||
Std dev (± x-fold) | 1.08 | 1.14 | 1.20 | 1.31 | 1.41 |