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Table 4 Expression stability values from the candidate reference genes calculated by the NormFinder software

From: Validation of reference genes for expression analysis in the salivary gland and the intestine of Rhodnius prolixus (Hemiptera, Reduviidae) under different experimental conditions by quantitative real-time PCR

R. prolixus s alivary glands

R. prolixus c rop

After blood feeding

After T. rangeli infection

After blood feeding

After T. rangeli infection

After T. cruzi infection

Ranking

Stability value*

Ranking

Stability value

Ranking

Stability value

Ranking

Stability value

Ranking

Stability value

ACT

0.118

GAPDH

0.152

TUB

0.039

TUB

0.045

ACT

0.054

GAPDH

0.125

TUB

0.176

GAPDH

0.072

ACT

0.109

GAPDH

0.127

TUB

0.144

ACT

0.181

18S

0.078

L26

0.172

TUB

0.130

18S

0.185

18S

0.202

L26

0.168

18S

0.174

18S

0.188

L26

0.350

L26

0.224

ACT

0.168

GAPDH

0.215

L26

0.377

Best combination

Stability value

Best combination

Stability value

Best combination

Stability value

Best combination

Stability value

Best combination

Stability value

GAPDH and ACT

0.087

TUB and ACT

0.077

GAPDH and TUB

0.043

TUB and ACT

0.074

GAPDH and ACT

0.077

  1. *The stability values are listed from the most stable to the least stable gene