Figure 3

A practical example using SpiroESTdb with "Electronic Northern". (a) The search interface. The steps involved in a "Electronic Northern" search are as follows: (a1) choose "Electronic Northern"; (a2) select search options for generating the appropriate output for your query, including "Annotation Algorithm", "Min.reads" and "Libraries". In the "Libraries" menu, the user can select a specific library; enter any additional information in the search field, if desired; and submit the query. (b) "Electronic Northern" results from a search for highly expressed genes. (b1) By selecting items under 'Acc.ID', detailed information can be obtained from the NCBI protein database. (b2) The number of reads can be displayed by double-clicking the center of the box in the corresponding color. (b3) The color spectrum indicates the expression level (the number of reads, n), ranging from low (black, n = 0) to high (red, n > 45).